In this answer, purity of ghee is tested by comparing home made ghee with store bought ghee. Store bought ghee is adulterated with three main ingredients. Learn about adulteration of ghee, types of adulterants, detection of adulterants in ghee. Buy unadulterated Pure Desi Ghee at VedicGiftShop. Detection of adulteration of ghee (clarified milk fat) with palm olein and sheep body fat using Reichert-Meissl (RM) value coupled with solvent.

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According to Ayurveda, ghee promotes intellect, is good for bone health and digestion of food. The absorbance of the Peak 1 remains almost same in all the samples while in Peak 2, the absorbance is the highest in ghee and steadily decreases with the increasing concentration of tallow in ghee and is the lowest in tallow.

Detection of tallow adulteration in cow ghee by derivative spectrophotometry

Unlike other oils, ghee is rich in butyric acid. National Center for Biotechnology InformationU. Add this mixed solution to about aeulteration ml of melted ghee. Test for adulteration with coconut oil Items required: After five minutes, if a red colour appears in the bottom of the ghee then the sample contains vegetable oil.

Controller of Publications, Government of India; Among reported techniques, ultraviolet fluorescence and paper chromatography technique are not that much sensitive while other methods require sophisticated instrumental facilities such as gas chromatography, mass spectrometry and costly analytical processes.

The Ayurvedic Pharmacopoeia adukteration India. The developed derivative spectroscopic method is a rapid, sensitive, cost-effective method for detection of tallow adulteration in cow ghee. Even then, ghee remained the preferred medium for special dishes on special occasions. The developed derivative spectrophotometric method is a rapid, sensitive, cost-effective method for gyee of tallow adulteration in cow ghee and has potential as a method for quantitative estimation of tallow in cow ghee.


Continued heating until the solids are adulyeration not burnt and turns a light brown colour. This decrease shows consistent linearity suggesting its applicability for quantitative estimation of tallow in cow ghee. It also contains other fat-soluble vitamins such as vitamin D and K2, which are usually lacking in the diet otherwise, and many of the minerals that are needed by our bodies.

An on-line differential converter, for obtaining the first derivative of absorption spectra. Detection of adulterants and their estimation is a key concern over many years.

Indicated values for commonly used parameters to differentiate pure and adulterated ghee materials are many a times overlapping. There is a need to develop a sensitive, rapid, cost-effective method. Hence, it will be useful in detecting and quantifying adulteration of tallow in cow ghee.

Detection of tallow adulteration in cow ghee by derivative spectrophotometry

Data was analyzed using data points each for slope calculation through PerkinElmer Data processor and viewer software to obtain second order derivative absorption adulreration.

Of course, it is important to ensure the purity of the ghee that you consume.

Let it stand for five minutes. It is adylteration that unrefined vegetable oils, old and rancid ghee, animal fats, coal tar dyes and other substances are mixed with ghee by unscrupulous elements to increase the quantity being sold. Ghee is one of the important dairy products in India and is normally prepared by using cow milk or buffalo milk.


Sent your message successfully! Now you have pure ghee ready. Nil Conflict of Interest: The value of the ratio of the absorbance of peaks at about nm and nm steadily decreases with the increasing proportion of tallow.

In another test tube mix 0. Test for adulteration with vegetable oil or margarine Items required: Jirankalgikar and Subrata De.

Application of paper chromatography to differentiate ghee from other fats: In recent years, however, research seems to indicate that ghee is better than many of the seed and vegetable oils that were used in its stead. Shake well, allow the mix to stand for 10 min. Test tube stand Procedure: Along with being rich in vitamins A, E and K, ghee also contains omega 3, 6 and 9 fatty acids which are good for the functioning of the heart and brain.

The milk should be whole and not skimmed. A test tube with a stopper or a small clear bottle with cap preferably sterilisedconcentrated hydrochloric acid, a small quantity of sugar.

Between these two peaks in each of adultetation spectra, one remains unchanged while the absorbance of another peak changes with the changing proportion of tallow in ghee. UV spectrophotometry The normal absorption spectra of ghee and tallow of all the samples were recorded in the range nm using a PerkinElmer UV-visible recording spectrometer Model — Lambda Today, the ideal diet is adupteration to be a combination of ghee and other oils so that the body can have the benefits of both.